PENGARUH PENAMBAHAN MINYAK ZAITUN DALAM PENGENCER KOMBINASI TRIS SARI BUAH SEMANGKA - KUNING TELUR TERHADAP KUALITAS SPERMATOZOA BABI LANDRACE

Abstrak

Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh penambahan minyak zaitun dalam pengencer kombinasi tris sari buah semangka - kuning telur terhadap kualitas spermatozoa babi landrace. Penelitian ini menggunakan metode eksperimen dari  rancangan acak lengkap dengan 5 perlakuan yaitu: P0= 40% Tris + 20%KT + 40% SBS + 0% MZ, P1= 40% Tris + 20%KT + 40% SBS + 3% MZ P2= 40% Tris + 20%KT + 40% SBS + 6% MZ, P3=40% Tris + 20%KT + 40% SBS + 9% MZ, P4= 40% Tris + 20%KT + 40% SBS + 12% MZ. Seluruh perlakuan disimpan di dalam cool box pada suhu 18-20°C. Evaluasi terhadap motilitas, viabilitas, abnormalitas dan daya tahan hidup spermatozoa dilakukan setiap 8 jam. Data dianalisis menggunakan software SPSS versi 25. Hasil penelitian yang diperoleh menunjukkan motilitas spermatozoa babi landrace yang disimpan selama 32 jam pada perlakuan P2 memiliki kualitas yang lebih tinggi  dibandingkan dengan perlakuan lainnya (P>0,05), motilitas (40,00%), viabilitas (42,50%), abnormalitas (3,50%) dan daya tahan hidup (32,00) jam. Disimpulkan bahwa penambahan 6% minyak zaitun dalam pengencer kombinasi tris sari buah semangka-kuning telur efektif dalam mempertahankan kualitas semen babi landrace

Abstract: This study aims to determine Effect Of Adding Olive Oil (Oo) In The Tris Combination Of Watermelon Juice (WMJ) - Egg Yolk (EY) Combination Diluent On The Quality Of Landrace Pig Spermatozoa.This research uses an experimental method from a completely randomized design with 5 treatments, namely: P0=40% Tris + 20%KT + 40% SBS + 0% MZ, P1=40% Tris + 20%KT + 40% SBS + 3% MZ P2 =40% Tris + 20%KT + 40% SBS + 6% MZ, P3=40% Tris + 20%KT + 40% SBS + 9% MZ, P4= 40% Tris + 20%KT + 40% SBS + 12 % MZ. All treatments were stored in a cool box at a temperature of 18-20°C. Evaluation of motility, viability, abnormalities and survival of spermatozoa is carried out every 8 hours. Data were analyzed using SPSS version 25 software. The results obtained showed that landrace pig spermatozoa motility stored for 32 hours in treatment P2 had higher quality compared to other treatments (P>0.05), motility (40.00%), viability (42.50%), abnormality (3.50%) and survival (32.00) hours. It was concluded that the addition of 6% olive oil in the tris watermelon juice-egg yolk combination diluent was effective in maintaining the semen quality of landrace pigs.